Determining CCS intensity profiles using growth rate distributions. (A) Trace clusters from test cells (excluded from the library) are matched with clusters from the CCS library by their growth rate histograms. Successful reproductions of the cluster traces occurred in multiple cell types and conditions. Two clusters and library matches are shown per condition. Intensity profiles of test clusters and corresponding growth rate distributions are shown in blue, and library matches are in red. Shaded regions in intensity profiles mark the standard deviation of the cluster. (B) In a U373 cell coexpressing fluorescently tagged AP2 and clathrin, the growth rate of AP2 is more peaked at low slope magnitudes (left). Corresponding library matches show continuing increase in clathrin signal after AP2 signal plateaued (right). (C) MβCD is added to BSC1 cells at t = 0. Growth rate distributions determined at different time points of the treatment are shown on the left. (right) Predicted intensity profiles for the given growth rate distributions. Gradual changes in growth rates result in increasing trace lifetime and peak intensity.