CCSs in proximity of substrate adhesion sites have reduced dynamics. (A) Arrowheads mark clathrin-coated plaques at the ventral surface of a BSC-1 cell stably expressing AP2-GFP. Bar, 5 µm. (B) Scatterplot shows maximum intensity versus the axial positions of CCSs detected at the ventral surface. The positions are relative to the lower bound of the confocal volume used for 3D tracking (Kural et al., 2012). CCS traces are divided into plaque (red) and pit (green and blue) populations based on their maximum intensities. Clathrin pits positioned closer to the substrate are in green (Ncells = 6 and Ntraces = 11,482). (C) Scatterplot shows the lifetime versus axial positions of the CCSs shown in B. (D) Growth rate distributions of the pit and plaque populations. The inset shows the distributions as bar plots.
