VAMP7 traffics with TYRP1 to maturing melanosomes upon BLOC-1 rescue. (a–t) BLOC-1^−/− (melan-pa) cells transiently transfected with GFP-VAMP7 (green), mCh-STX13 (red), and either myc-Pallidin (BLOC-1^R) or myc-Muted (BLOC-1^−/−C) were fixed 12 or 24 h after transfection. Cells were then immunolabeled for TYRP1 (blue, a–l) or PMEL (blue, m–t) and analyzed by deconvolution immuno-FM. Arrowheads show GFP-VAMP7 and TYRP1 (a–l) or GFP-VAMP7 alone (m–t) retained in mCh-STX13–labeled endosomes, and arrows point to GFP-VAMP7 and either TYRP1 (a–l) or PMEL (m–t) colabeled in melanosomal precursors that lack mCh-STX13. Insets are boxed regions magnified five times. Bars: (main) 10 µm; (insets) 2 µm.