Tissue-wide quantitation of mitophagy in vivo. Representative images of skeletal muscle (A), liver (B), and spleen (C) used to perform generalized analysis of mammalian mitophagy across selected tissues in vivo. (D) Scatterplot depicting the mean relative level of global mitophagy in different organs in vivo, where each data point represents an organ from an individual animal and error bars represent standard error. Bars, 20 µm.