Mitophagy and mitochondrial architecture in defined subsets of cerebellar neurons. (A) Representative images from a parasagittal section of adult cerebellum from mito-QC reporter mouse, labeled with antibodies to the Purkinje neuron marker calbindin-D28K. ML, molecular layer; GCL, granule cell layer; V, vasculature. (B) Reporter signal in same immunolabeled region as A, with DAPI shown in blue. (C–F) Magnified view of a calbindin-positive Purkinje neuron in the cerebellum, with panels showing calbindin (C), mCherry (D), GFP (E), and merge (with DAPI; F). Dashed lines demarcate calbindin-positive neuronal processes, whereas arrows/asterisks highlight mitochondria (mCherry- and GFP-positive) situated at dendritic bifurcation points and arrowheads highlight examples of mitophagy (mCherry only) in the soma. Bars, 20 µm.