The peripheral structure containing ciliary markers is a postmitotic midbody. (A) The images correspond to 3D reconstructions of cells expressing cherry-tubulin that were filmed during cell division. The images were pseudocolored based on height, using the color scale on the left, to highlight that the intercellular bridge forms at the top of the cells and that the postmitotic midbody remnant localizes after abscission at a peripheral position at the apical surface. The arrowhead points to the postmitotic midbody. Bar, 5 µm. An enlargement of the boxed region at 0 and 80 min is also shown. Bars, 2 µm. (B) Serial EM sections of the apical region of MDCK cells during cytokinesis. The sections are numbered S1 onwards from the lower section to the top. Note the progressive loss of microvilli at the top sections. Bar, 1 µm. (C) Cells expressing dsRed-centrin were stained for IFT20 and PRC1. The enlargement of the boxed region shows the fluorescent signal for IFT20 and PRC1. The dashed line indicates the cell contour. The white and green arrowheads point to the centrosome and the peripheral structure, respectively. Bars, 2 µm. (D) EM micrograph of a cell with a peripheral midbody remnant (left panel) and enlargements of different serial sections (S3, S4, and S7, right). Note that in some of the sections it appears that the remnant is connected to the rest of the cell by a thin tether (empty arrowhead) as shown in the enlargements of the boxed regions of sections S3, S4, and S7 (S3′, S4′, and S7′, bottom). Asterisks indicate cell junctions. The images are orthogonal serial sections from the same cell. The sections were numbered S1 onwards from the back to the front. Bars: (panoramic view) 3 µm; (enlargements) 500 nm.