Cortactin does not control exosome cargo selection. (A) Classification of proteins identified in equal numbers of exosomes that are common between SCC61-control (Sc), cortactin-KD1 (KD1) and cortactin-KD2 (KD2) cells based on their UniProt identified function. (B) Venn diagram representing the presence, absence, or overlap of proteins identified through LC-MS/MS in exosomes purified from the indicated cell lines. Proteomics data n = 3 independent experiments. (C) WB analysis of proteins present in an equal number of exosomes purified from control and cortactin-KD cells. (D) Quantitation of WB. n = 3. Bar graphs = mean ± SE. Significance analyzed by Student’s t test. ns, not significant. (E) WB analysis of MMP2 in whole-cell lysate (WCL), conditioned media (CM), microvesicle (MV), exosomes (UC exo), and supernatant (Sup) from the UC exo centrifugation step. Representative of n = 3 experiments.