The anaphase cortex is uniformly responsive to active RhoA. (A) Images of noncontractile anaphase HeLa cells (200 nm BI 2536) expressing PR_GEF photoactivated (yellow boxes) parallel to the spindle axis. Quantification of the extent of ingression (B) and constriction rate (µm/min; C) of light-induced furrows at cell midzone and poles. (D) Images of noncontractile anaphase HeLa cells (200 nm BI 2536) expressing PR_GEF photoactivated at both the midzone and polar regions. Comparison of the extent of ingression (%; E) and constriction rate (µm/min; F) at the midzone versus pole for individual cells (n = 8). (G) Images of noncontractile anaphase HeLa cells expressing PR_GEF photoactivated with pulses of the indicated duration at the midzone and pole. (H) Images of HeLa cell expressing mApple-Actin undergoing cytokinesis (−BI 2536). (I) Images of HeLa cell expressing PR_GEF undergoing cytokinesis (−BI 2536) with polar photoactivation (n = 5). (J) Ingression kinetics of the endogenous furrow with (red) and without (gray) polar photoactivation in a representative cell. In H and I, cells were photoactivated for 30 min. Bar, 5 µm.