Figure 1.
Figure 1. Light-mediated activation of RhoA. (A) Schematic depicting the pathway that promotes RhoA activation during cytokinesis. (B) TULIPs-mediated activation of RhoA by light-directed recruitment of PR_GEF. Photoactivation of NIH3T3 cells (yellow boxes) induces local recruitment of PR_GEF (n = 9; C), F-actin polymerization (n = 7; E), and myosin accumulation (n = 15; G). Quantification from representative cells of the relative increase in intensity in the activation region (magenta) vs. a control region (black) for PR_GEF (D), mApple-actin (F), and mCherry-MLC (H) over time. During photoactivation (blue box), cells were locally illuminated (405 nm) with a 960-ms pulse every 20 s. PR_GEF or effectors were imaged every 20 s. a.u., arbitrary units. Bars, 10 µm.

Light-mediated activation of RhoA. (A) Schematic depicting the pathway that promotes RhoA activation during cytokinesis. (B) TULIPs-mediated activation of RhoA by light-directed recruitment of PR_GEF. Photoactivation of NIH3T3 cells (yellow boxes) induces local recruitment of PR_GEF (n = 9; C), F-actin polymerization (n = 7; E), and myosin accumulation (n = 15; G). Quantification from representative cells of the relative increase in intensity in the activation region (magenta) vs. a control region (black) for PR_GEF (D), mApple-actin (F), and mCherry-MLC (H) over time. During photoactivation (blue box), cells were locally illuminated (405 nm) with a 960-ms pulse every 20 s. PR_GEF or effectors were imaged every 20 s. a.u., arbitrary units. Bars, 10 µm.

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