Multiple annexins contribute to repair cap formation. (A) Annexins A1, A2, A5, and A6 were rapidly recruited to the site of laser wounding and were seen between 4 and 14 s after membrane disruption. With each annexin repair cap, an annexin free repair zone was visible within the myofiber (n ≥ 14 myofibers from n ≥ 5 mice per condition). Bar, 4 µm. (B) Fluorescence recovery after photobleaching (FRAP) was performed with GFP-tagged annexins. The diffusion of the different annexins over the first 20 s after injury was comparable (n ≥ 7 myofibers from n = 3 mice per condition). (C) Kinetics for GFP-tagged annexins showed a rapid increase of annexin at the lesion reflecting recruitment to the repair cap (*, P < 0.05; n = 5 myofibers from n = 3 mice per condition). Error bars represent SEM.