Effect of confinement width and cluster size. (A) Schematic illustration and examples of maximal projection of a Z-confocal stack used to measure the number of cells in NC streams of different widths. White line illustrates how the stream width was measured. Width (W) and number of cells (N) are shown at the bottom, with SD in parentheses; ne, number of embryos analyzed. (B) Snapshots of simulations with increasing confinement widths (w, cell diameters) and cluster size (N, number of cells). (C and D) Cluster persistences and transport ratio (percentage of cells in the cluster migrating at least 150 µm away from the cluster’s edge in 5 h). Heatmaps represent median values from n = 50 simulations. Dots show experimental values for NCs migrating in vivo in zebrafish and Xenopus embryos. Numbers on dots correspond to the same numbers shown in A.