Figure 2.
Figure 2. Versican is required for normal NC migration. (A and B) qPCR (A) and Western blot (B) analysis of CoMO- and VsMO-injected embryos. (C–E) ISH of Twist in embryos injected with either CoMO (C) or VsMO (D), and inhibition of migration (E; C: n = 91, D: n = 135). (F and G) Graft experiments: VsMO + fluorescein-dextran (FDx)–injected NCC grafted into control host (F; 76% of migration, n = 17) or CoMO + FDx NCC grafted into VsMO-injected host (G; 13% of migration, n = 15). (H and I) ISH against Krox20, showing an uninjected versus VsMO-injected side of a stage 24 embryo (H, dorsal view; n = 53), and number of ectopic NCC (I; n = 51). Bars, mean; error, SEM. Arrowhead represents a migrating NC. **, P < 0.01.

Versican is required for normal NC migration. (A and B) qPCR (A) and Western blot (B) analysis of CoMO- and VsMO-injected embryos. (C–E) ISH of Twist in embryos injected with either CoMO (C) or VsMO (D), and inhibition of migration (E; C: n = 91, D: n = 135). (F and G) Graft experiments: VsMO + fluorescein-dextran (FDx)–injected NCC grafted into control host (F; 76% of migration, n = 17) or CoMO + FDx NCC grafted into VsMO-injected host (G; 13% of migration, n = 15). (H and I) ISH against Krox20, showing an uninjected versus VsMO-injected side of a stage 24 embryo (H, dorsal view; n = 53), and number of ectopic NCC (I; n = 51). Bars, mean; error, SEM. Arrowhead represents a migrating NC. **, P < 0.01.

This Feature Is Available To Subscribers Only

or Create an Account

Close Modal
Close Modal