Cep97 functions downstream of Asl in mGSC centriole length control. (A) Centriole targeted Cep97 construct. LRR, leucine-rich repeat; PACT, centriole targeting domain of PLP. (B) A representative centriole in asl mGSCs expressing Cep97^PACT. PLP, blue; Cep97^PACT, green; Ana1::tdTomato, red. Linescans (dashed line) along the long axis of the centriole, normalized to peak intensity. (C) A representative centriole in asl mutant mGSCs. PLP, blue; Ana1::tdTomato, red. Linescans as in B. (D) Length of Ana1::tdTomato in asl mutant mGSC centrioles at indicated developmental time from individuals with or without Cep97^PACT expression. Additional examples are shown in Fig. S4. Error bars are ±SD. Comparison: unpaired t tests with Welch’s corrections when appropriate. N, centrioles measured. Bars, 1 µm.