Asterless and Cep97 interact. (A) Schematics of the subfragments (F1–F3) of Asl and Cep97 used for interaction assays. Red numbers refer to B. Numbers are amino acids. Lines, Y2H interactions; CC, coiled coil; LRR, leucine-rich repeat. (B) Asl F3 interacts with Cep97 F1 (1) and Cep97 F3 (2) by Y2H screening. The first column shows the presence of Y2H plasmids. The remaining columns show interaction under conditions of increasing stringency (see Materials and methods). Red numbers refer to A. (C) GFP::Asl F3 coIPs Flag::Cep97-F1 from S2 cells. (D) GFP::Asl F3 coIPs Flag::Cep97-F3 from S2 cells. (E) GFP::Asl full-length coIPs Flag::Cep97 full length from S2 cells. (F) GFP::Cep97 full-length coIPs Flag::Asl full length from S2 cells.