Asl controls centriole length in female germline and somatic cells. (A–G) Remnant centrioles in wt (aslmecD/TM6B) and asl fGSCs from PAs. (A) Cell types in Drosophila ovaries (centrioles, red). (B) Remnant centrioles (Ana1::tdTomato, red; PLP, green) in asl fGSCs are longer than in wt. Arrowheads, centrioles in fGSCs. DAPI, blue. Boxes are enlarged in D. (C) Measurements of centriole length (Ana1::tdTomato), in fGSCs. (D) Boxed region from B. PLP is along the length of asl centrioles. (E) GFP::Sas-6 (green) extends the length of asl centrioles. (F and G) GFP::Cep97 (F, green) and CP110::GFP (G, green) localize to the proximal tip of asl fGSC centrioles. (H–J) Centrioles in wt (aslmecD/TM6B) and asl larval wing discs. (H) Remnant asl centrioles (Ana1::tdTomato, red) are longer than wt. PLP (red) is along their entire length. (I) GFP::Sas-6 (green) extends the entire length of asl centrioles. (J) Centriole length (Ana1::tdTomato) in wing discs. Mean ± SD, red. Comparison: unpaired t tests, with Welch’s correction. ****, P < 0.0001. Bars: (B) 5 µm; (D–I) 1 µm.
