Asl is required for proper centriole length control. (A) asl embryos receive maternal Asl protein and mRNA (purple) used for the first 2 h of development. When this protein runs out, centriole duplication halts. Centrioles present at this point are reliably inherited by the developing testes (remnant centrioles). (B) Cell types in the Drosophila testis (centrioles, red). (C) Remnant centrioles (Ana1::tdTomato, red) in larval asl mGSCs do not have Asl (green) and are longer than in wt. Arrowheads, mGSC centrioles. Boxed region enlarged. DAPI, blue. (D) Centriole length (Ana1::tdTomato length) in mGSCs in wt (aslmecD/TM6B) and asl mutants in larvae 72 h after egg laying (L72), larvae 96 h after egg laying (L96), or PAs. Mean ± SD, red. Comparison: ordinary one-way analysis of variance followed by Tukey’s multiple comparisons test. ****, P < 0.0001. (E) Representative centrioles in mGSCs at indicated stages. The longest centriole observed is on the right. Asterisk, centriole in adjacent hub cell. Bars: (C) 5 µm; (insets) 1 µm; (E) 1 µm.
