Schematic summary of MT sliding scenarios in experiments on migratory neurons and the effects on neuronal morphology, migration, and MTs in the leading process. Neurons treated with control siRNA display a minimal amount of sliding (green MT = sliding MT), tortuosity, and MT buckling and migrate in a smooth, consistent path (straight green arrow). When FCPT is used to prevent MT sliding (represented by X), MTs in the leading process of control siRNA-treated neurons show an increase in MT buckling (but do not become tortuous), and their migration path is irregular (curved green arrow). After treatment with ninein siRNA, MT buckling is observed, the leading process becomes tortuous, and migration is compromised (small green arrow). When FCTP is applied to the ninein-depleted neurons, MT buckling and tortuosity are exacerbated, and neuron movement is restored (but with abnormalities in migration path).