RhoB is expressed in intestinal vessels of patients with Crohn’s disease and hepatic sinusoids. (A) Moderate immunohistochemical staining of RhoB from human umbilical veins. (B–D) Large intestine from a patient with Crohn’s disease showing intense endothelial RhoB staining (arrows) in inflamed vessels containing high levels of adhered immune cells. (C and D) Differential RhoB staining, which suggests increased RhoB expression in vessel areas with high levels of leukocyte adhesion and capillary hyperplasia (arrows), with respect to areas with no immune cells (arrowheads; see Fig. S2). (E–G) Consecutive tissue sections showing that RhoB is preferentially expressed in small vessels positive for smooth muscle actin (SMA) and negative for the NG2 proteoglycan. (H and I) RhoB staining in sinusoids from a control liver (H) and hepatitis B virus–infected liver (I). Bars: (A, B, and E–I) 100 µm; (A, inset, and C and D) 10 µm.