Multiple afadin domains contribute differentially to different aspects of its function. (A) Diagram of afadin and domain deletion mutants used. (right) Summary of their effectiveness at rescuing different aspects of afadin function. (B–I) Representative fields of ZO KD, ZO/afadin KD, or ZO/afadin KD cells rescued by indicated afadin mutants. Arrows, small gaps in ZA at TCJ/MCJs; asterisks, larger ZA gaps. Projections of apical 3-µm sections. (J) Closeup showing progressively larger gaps in ZA. (K) Quantification of major/minor axis aspect ratio. Error bars are SDs of >900 cells (n = 3). (L and M) Quantification of large (L) or small (M) ZA gaps. Error bars are SDs of each genotype.