Requirement for MVP and Rab11b in cancer metastasis. (A–D) Lung metastases of MDA-MB-231 cells expressing a luciferase reporter were measured after their injection into the tail veins of nude mice. Cells were transfected with an shRNA plasmid (to silence Rab11b) or a control vector (Scramble; A and B) or were pretreated with Simvastatin or a control solution for 2 d before the injection (C and D). In C and D, mice received Simvastatin or a control solution, as indicated, every day after the injection. In A and C, bioluminescence intensities from the chests of the injected mice were measured on the indicated days. Results are shown as mean ± SEM. n = 5. *, P < 0.05. Representative images of the mice are shown on the right. In B and D, representative whole images of the lungs (top) and hematoxylin and eosin staining of lung sections (bottom) 28 d after the injection are shown. Bars, 100 µm.