Figure 5.
Figure 5. Requirement for MVP and Rab11 in the PM recruitment and activation of Arf6 in HMLE cells. (A) Arf6 activities of cells pretreated with siRNAs for Rab11a, Rab11b, Rab11c, or Irr and maintained in their growth medium were measured. (B and C) Subcellular localization of Arf6-EGFP or Arf6-mCherry was examined in cells pretreated with siRNAs for Rab11b or Irr (B) or preincubated with Simvastatin or DMSO for 24 h (C) and maintained in growth medium. F-actin was visualized using Alexa Fluor 568– (B) or Alexa Fluor 488–conjugated (C) phalloidin. Fluorescence intensities along the green arrows (B) and the red arrows (C) are shown on the right. Each experiment was performed at least twice, and representative images from >10 cells then examined are shown. Bars, 10 µm. a.u., arbitrary units.

Requirement for MVP and Rab11 in the PM recruitment and activation of Arf6 in HMLE cells. (A) Arf6 activities of cells pretreated with siRNAs for Rab11a, Rab11b, Rab11c, or Irr and maintained in their growth medium were measured. (B and C) Subcellular localization of Arf6-EGFP or Arf6-mCherry was examined in cells pretreated with siRNAs for Rab11b or Irr (B) or preincubated with Simvastatin or DMSO for 24 h (C) and maintained in growth medium. F-actin was visualized using Alexa Fluor 568– (B) or Alexa Fluor 488–conjugated (C) phalloidin. Fluorescence intensities along the green arrows (B) and the red arrows (C) are shown on the right. Each experiment was performed at least twice, and representative images from >10 cells then examined are shown. Bars, 10 µm. a.u., arbitrary units.

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