Figure 2.
Figure 2. Mutant p53 is crucial for ligand-induced Arf6 activation but not for the trans-activation of c-Met. (A and B) Arf6 activation (A) and Matrigel invasion (B), induced by EGF, HGF, or TGFβ1, as indicated, were measured in MDA-MB-231 cells (parental) and their p53 derivatives. Exogenous p53 proteins were tagged with V5. Matrigel invading cells were quantified as described in the Matrigel invasion assay section within Materials and methods. (C) TGFβ1 induces c-Met activation irrespective of p53 status. Activation of c-Met upon 5 min TGFβ1 stimulation of MDA-MB-231 cells and their p53 derivatives was detected using the pY1234/1235 antibody. (D and E) HGF-induced Arf6 activation (D) and Matrigel invasion activities (E) of MDA-MB-435s cells, Hs578T cells, and their shp53 cells were measured. Matrigel invasion activities were measured as in B. The results represent mean ± SEM. n = 3. *, P < 0.001. Total, total cell lysate.

Mutant p53 is crucial for ligand-induced Arf6 activation but not for the trans-activation of c-Met. (A and B) Arf6 activation (A) and Matrigel invasion (B), induced by EGF, HGF, or TGFβ1, as indicated, were measured in MDA-MB-231 cells (parental) and their p53 derivatives. Exogenous p53 proteins were tagged with V5. Matrigel invading cells were quantified as described in the Matrigel invasion assay section within Materials and methods. (C) TGFβ1 induces c-Met activation irrespective of p53 status. Activation of c-Met upon 5 min TGFβ1 stimulation of MDA-MB-231 cells and their p53 derivatives was detected using the pY1234/1235 antibody. (D and E) HGF-induced Arf6 activation (D) and Matrigel invasion activities (E) of MDA-MB-435s cells, Hs578T cells, and their shp53 cells were measured. Matrigel invasion activities were measured as in B. The results represent mean ± SEM. n = 3. *, P < 0.001. Total, total cell lysate.

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