The mother centriole is transported and anchored to the cell membrane shortly after PBI extrusion. (A) pmOdf2-mEGFP labels mother centrioles; EB3-mCherry3 labels microtubule plus ends. The left panel shows maximum intensity projections for selected time points, the right panel shows the region around the centriole for all frames. Bars: (left) 5 µm; (right) 1 µm. (B) pmPoc1-mEGFP imaged in 3D at high temporal resolution (see Video 4). 3D volume rendering of the data overlaid with an isosurface reconstruction of the cell outline (gray). Identified positions of centrioles are shown as green and pink spheres for mother and daughter centrioles, respectively. z-Stacks were acquired every 12 s. Bar, 5 µm. (C) Example of retrieved 3D coordinates of a mother centriole and the closest point on the plasma membrane. Time point selected from the dataset in B. (D) Example of a 3D mother centriole trajectory during the transport phase showing the linear fit (dashed line) to estimate the speed of motion. (E) Plot of centriole distance from the plasma membrane over time for the oocyte shown in B. (F) Dashed squares indicate the area shown in insets (see Video 5). Asterisk in the first frame shows nuclear position after centrifugation. t = 0 is shortly after NEBD. 3D rendering as in B. Bars: (main) 40; (inset) 5 µm. (G) Distance measurements of mother and daughter centrioles to the plasma membrane over time for the oocyte shown in F. M1 and M2 are mother centrioles; D1 and D2 are daughter centrioles identified based on their microtubule nucleating activity at the end of meiosis. (H) Centrioles were tracked in an oocyte expressing pmPoc1-mEGFP and hsEB3-mCherry3 starting from anaphase I onset (only pmPoc1-mEGFP is shown). 3D rendering as in B. Bar, 5 µm (see Video 6). (I) Plot of centriole distance from the plasma membrane over time for the oocyte shown in H.