Mother centrioles are invariably extruded into the polar bodies, leaving a single daughter centriole in the mature egg. (A–C) Oocytes injected with and expressing the indicated fluorescent markers were imaged by 3D confocal microscopy throughout meiosis, starting at metaphase I. Overview images are maximum intensity projections of the entire z-stacks; insets are single confocal sections of the regions marked on the overviews. Here and in other figures, unless otherwise indicated, the dashed line indicates the oocyte contour based on the transmitted light image (not depicted). z-Stacks were acquired every 30–60 s, and time is shown in mm:ss. Bars: (overview images) 5 µm; (insets) 1 µm. (A) pmPoc1-mEGFP labels centrioles and microtubules (see Video 1). (B) pmOdf2-mEGFP specifically labels mother centrioles; Cy3-tubulin labels microtubules (see Video 2). (C) hsCentrobin-mEGFP labels daughter centrioles; pmPoc1-mCherry labels centrioles and microtubules (see Video 3). (D) Distribution of mother and daughter centrioles after PBII extrusion determined using the indicated centriolar markers. In all oocytes examined, the two mother centrioles were extruded into the polar bodies (configuration I); extrusion of the daughter centriole into PBII (configuration II) was never observed. An Odf2-mEGFP–expressing oocyte, coinjected with Cy3-Tubulin, is shown as an example on the left (maximum intensity projection of a z-stack; processed to remove autofluorescence as described in Materials and methods). Bar, 5 µm.