Arl2 interacts with Mts and has a conserved role in regulating microtubule growth. (A) Coimmunoprecipitation of HA-Mts and different forms of Arl2-Venus. (B) Metaphase NBs of control, Arl2^T30N overexpression, and Arl2^T30N overexpression in mts^XE2258/+ background labeled for aPKC, PH3, and DNA. (C) NBs from arl2^Δ156 and arl2^Δ156 with overexpression of mouse Arl2 (mArl2) labeled for α-tubulin, GFP, and DNA. NB outlines are indicated by white dotted circles. The arrow indicates microtubule asters. (D) NB clones of arl2^Δ156 and arl2^Δ156 with mArl2 overexpression labeled for Dpn, Ase, and CD8. The NB lineages are labeled by CD8-GFP and are indicated by white dotted lines. Arrows indicate NBs. (E) A working model. Bars, 5 µm.