![Figure 3. Arl2 promotes microtubule growth. (A) Control and Arl2T30N NBs were stained for α-tubulin (α-tub) and DNA after recovery from treatment on ice. (B) Quantification of paused EB1-GFP particles bound to centrosomal microtubules. Control: 16.4% (interphase [Int], n = 140), 16.5% (prophase [Pro], n = 133), 15.0% (metaphase [Meta], n = 133); Arl2T30N: 34.2% (interphase, n = 146), 15.8% (prophase, n = 133), 15.9% (metaphase, n = 138). See also Table S2. (C) Interphase and prometaphase/metaphase NBs in control (FRT82B), arl2Δ156, and arl2Δ309 MARCM clones labeled for CNN, α-tubulin, CD8, and DNA. (D) Control and Arl2Q70L NBs labeled for Insc, α-tubulin, and DNA or for CNN, α-tubulin, and DNA. Arrowhead, DNA mass of a polyploidy cell. Arrows, centrosomes. (E) α-Tubulin and DNA staining in control and Arl2Q70L metaphase NBs from microtubule regrowth assay. Cell outlines are indicated by the white dotted lines. Bar, 5 µm.](https://cdn.rupress.org/rup/content_public/journal/jcb/212/6/10.1083_jcb.201503047/6/jcb_201503047_fig3.jpeg?Expires=1771625678&Signature=NLx7m-hi~nXIABQkjcb3LQgvKMqsr8s~HA9SCTXtSHZB~EmrrBjJ2RbSVrfGBecLQf6xShLC8DKpJLSPT87ixngJpkStMMoUggLvTOqDbw24MBAsXFpavSy93NScddHFGbvf8ug56zIR7OfYHuNFZhxj6mApf6mXvqt3Px5QyMR0BYIUDmDasgx8cWiutmomFz3nMsaxgRaiPiezbeSRjJy28IETjJuHJTOA86vTJ-pmgef2Uu2fPjqP6zYP2U~QSZZIE-nSMO95SrUzDlwrASxuEYWQH5BuobeWxHEODjbsHzndInqYPP-XqAVaRSE~1WZq7jrUnOp~2T6kQL~KbA__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Arl2 promotes microtubule growth. (A) Control and Arl2T30N NBs were stained for α-tubulin (α-tub) and DNA after recovery from treatment on ice. (B) Quantification of paused EB1-GFP particles bound to centrosomal microtubules. Control: 16.4% (interphase [Int], n = 140), 16.5% (prophase [Pro], n = 133), 15.0% (metaphase [Meta], n = 133); Arl2T30N: 34.2% (interphase, n = 146), 15.8% (prophase, n = 133), 15.9% (metaphase, n = 138). See also Table S2. (C) Interphase and prometaphase/metaphase NBs in control (FRT82B), arl2Δ156, and arl2Δ309 MARCM clones labeled for CNN, α-tubulin, CD8, and DNA. (D) Control and Arl2Q70L NBs labeled for Insc, α-tubulin, and DNA or for CNN, α-tubulin, and DNA. Arrowhead, DNA mass of a polyploidy cell. Arrows, centrosomes. (E) α-Tubulin and DNA staining in control and Arl2Q70L metaphase NBs from microtubule regrowth assay. Cell outlines are indicated by the white dotted lines. Bar, 5 µm.
