Arl2 regulates the asymmetric division of NBs. (A) Metaphase NBs of control, arl2^Δ156, arl2^Δ309, and Arl2^WT overexpression in arl2^Δ309 MARCM clones and control, arl2^GS17851, and Arl2^WT overexpression in arl2^GS17851 MARCM clones labeled for aPKC, CD8, PH3, and DNA. (B) Quantification of aPKC localization in A. In all control metaphase NBs, aPKC displayed strong apical crescents (n = 38). Only 30% (arl2^Δ156, n = 50), 25% (arl2^Δ156, n = 52), and 28% (arl2^GS17851, n = 46) metaphase NBs had aPKC crescent. (C) Telophase NBs of control and arl2^Δ309 MARCM clones labeled for aPKC, CD8, PH3, and DNA. (D) Metaphase NBs of control, Arl2^T30N, arl2^Δ156/+ (overexpressed Arl2^T30N, arl2^Δ156/+), Arl2^T30N and arl2 RNAi (GD44334) KD labeled for Insc, α-tubulin, and DNA. (E) Telophase NBs of control, Arl2^T30N, and Arl2^T30N, arl2^Δ156/+ (overexpressed Arl2^T30N, arl2^Δ156/+) labeled for aPKC, phalloidine (Phall) and DNA. D1 and D2 indicate the diameters of the NB daughter and GMC daughter, respectively. (F) Quantification of the ratio of D1 to D2 for E: control, 2.10 ± 0.24 (n = 55); Arl2^T30N, 1.65 ± 0.29 (n = 47); Arl2^T30N, arl2^Δ156/+: 1.51 ± 0.19 (n = 54). Mean and SEM are shown. ***, P < 0.001. (G) Time-lapse imaging of control (G147-GFP/+) and Arl2^T30N NBs expressing G147-GFP.