Distribution change of cadherin-catenin complex in the developing mouse OE and the phenotypes of αN-catenin KO mice. (A) Immunostaining for β-catenin in the developing mouse OE. Insets show immunostaining for ZO-1 (red), which indicates all boundaries. A representative image is shown of six independent experiments. The graph notations are the same as those in Fig. 3 A. (B) The cellular patterns in the OE derived from WT or αN-catenin KO mice on P0. The immunostaining for ZO-1 is shown. Arrowheads indicate attached OCs. (C) Statistical analysis of αN-catenin-KO phenotypes on P0. Top: number of SCs. Middle: number of OCs. Bottom: number of aberrant attached OCs. Results shown are the mean ± SD; *, P < 0.001. n = 9. (D) Immunostaining for β-catenin in the OE of WT (left) or αN-catenin KO (right) mice on E14 and E16. Insets show immunostaining for ZO-1 (red). A representative image is shown of six independent experiments. The graph notations are the same as those in Fig. 3 A. (E) Statistical analysis for the normalized fluorescence intensity of β-catenin in the OE of WT (left) or αN-catenin KO (right) mice on E14 and E16. Results shown are the mean ± SD; *, P < 0.001. n = 9.