Figure 9.
Figure 9. Rotating MCF10A acini form polarized actin-rich protrusions. MCF10A cells expressing LifeAct-EGFP were grown in a 3D basement membrane matrix for 2 d. (A) Confocal sections of a MCF10A acinus were taken at 3-min intervals as indicated. The LifeAct-EGFP signals are shown as maximum intensity projections from images acquired from the upper half of the acinus (see also Video 10 A for a 3D presentation of LifeAct-EGFP localization at time point 0). Arrowheads indicate dynamic actin protrusions. Actin dynamics recorded over a period of 40 min are shown in Video 10 B. Bars, 5 µm. (B) Schematic representation of a rotating MCF10A acinus. White arrows indicate the direction of rotation, and actin protrusions are depicted in red. Note that tricellular corners are highly enriched for actin protrusions.

Rotating MCF10A acini form polarized actin-rich protrusions. MCF10A cells expressing LifeAct-EGFP were grown in a 3D basement membrane matrix for 2 d. (A) Confocal sections of a MCF10A acinus were taken at 3-min intervals as indicated. The LifeAct-EGFP signals are shown as maximum intensity projections from images acquired from the upper half of the acinus (see also Video 10 A for a 3D presentation of LifeAct-EGFP localization at time point 0). Arrowheads indicate dynamic actin protrusions. Actin dynamics recorded over a period of 40 min are shown in Video 10 B. Bars, 5 µm. (B) Schematic representation of a rotating MCF10A acinus. White arrows indicate the direction of rotation, and actin protrusions are depicted in red. Note that tricellular corners are highly enriched for actin protrusions.

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