EEs colocalize with PxdA and moving peroxisomes. (A) Representative kymographs generated from simultaneous time-lapse movies of PxdA-mKate and GFP-RabA/5a–labeled EEs. (B) Bar graph quantifying the colocalization of PxdA-mKate and EEs. EEs colocalized with 54.8% ± 3.8% (SEM) PxdA puncta (n = 20 kymographs from 10 cells). PxdA colocalized with 89.7% ± 2.0% EEs (n = 20 kymographs from 10 cells). (C) Representative micrographs of PxdA-mKate distribution in WT versus hookAΔ hyphae. (D) Normalized line scans of WT and hookAΔ hyphae (P < 0.0001, two-way analysis of variance, Bonferroni post hoc test significant between 0.64 and 1.92 µm; n = 5 hyphae per genotype). (E) A kymograph generated from a simultaneous time-lapse movie of peroxisomes (pex) and EEs. 71.4% of moving peroxisomes (n = 28) overlapped with EEs. Bars: (x axis) 5 µm; (y axis) 5 s. (F) Stills from Video 8 of peroxisomes (Pex) and EEs. White arrows point to a co-migrating EE and peroxisome. Bar, 5 µm. (G) Normalized intensity line scans of peroxisomes and EEs during comigrating runs (n = 11).