Endosomal PtdIns3P persists in sorf-1 or sorf-2 mutants when Rab switch genes are deleted. (A–C) Dynamics of PtdIns3P (marked with 2xFYVE::GFP) in sand-1(ok1963) (A), rab-7(ok511) (B), and tbc-2(tm2241) (C) mutants. Arrows indicate disappearance of 2xFYVE::GFP from endosomes after injection of TR-BSA. (D and E) Time-lapse imaging of endosomal 2xFYVE::GFP in coelomocytes in rab-7(ok511) mutants and double mutants of rab-7 with sorf-1 or sorf-2 (D) and in sand-1(ok1963) mutants and double mutants of sand-1 with sorf-1 or sorf-2 (E). (F) Duration of endosomal 2xFYVE::GFP (mean ± SEM) in the indicated genotypes. EEs, early endosomes. (G) Disappearance rate of 2xFYVE::GFP-positive vesicles. ≥16 coelomocytes were scored per genotype. ***, P < 0.001.