Sentin function, not pole localization, depends on EB1 interaction. (A and B) Immunostaining of mature oocytes. (C) Localization of GFP-Sentin at different stages of oocytes (n = 13, 5, and 12). Error bars indicate the 95% confidence intervals. *, P < 0.05; ***, P < 0.01, significant difference from early stage 13. (D) Time-lapse images of oocytes expressing GFP-Sentin and Rcc1-mCherry. The numbers indicate minutes from nuclear envelope breakdown. (E) Live wild-type control oocyte expressing GFP-tagged full-length Sentin and Sentin(1–840) lacking the EB1-interacting domain. (F) The distances between homologous centromeres (cen 3) in mature oocytes from wild-type control and sentin mutants without a transgene (–) and with transgenes expressing full-length Sentin and Sentin(1–840). ****, P < 0.001, significant difference from the control. (G) Immunoblots of adult females from strains shown in F probed with antibodies against Sentin (top) and α-tubulin (bottom). (H) A model of Sentin action. Bars, 10 µm.