Prolonged GFA1 mRNP interactions with the NE in mex67-5. (A) Dot plot displaying the length of time a GFA1 mRNP persists in a single state (nuclear docked, transition, or cytoplasmic docked) in REF (n = 49, 82, and 108) and mex67-5 (n = 34, 94, and 151) strains. Note that the data presented here use all trace data where a particle interacted with the NE independent of the trace resulting in mRNP export. (B) Selected nonconsecutive frames show the persistent interaction of GFA1 mRNPs with the NE in mex67-5 cells (see Video 7). Cells were imaged at 26°C and 67 Hz with the time from the start of the event given in the bottom right of each image in milliseconds. The last image and inset show an overlay of the mRNP path that is color coded based on position (gray, nuclear docked; yellow, transition; blue, cytoplasmic docked/cytoplasm). Bars, 1 µm.