GFA1 mRNP export and NE scanning in REF strain. (A) Merged and registered images show consecutive frames of a successful export event based upon tracking of the tagged GFA1 mRNP across the NE in the REF strain (see Video 2). (B and C) Selected nonconsecutive frames show local (B) and distributive (C) NE scanning interactions between an mRNP and the NE (see Videos 5 and 6, respectively). For all panels, cells were imaged at 26°C and 67 Hz with the time from the start of the event given in the bottom right of each image in milliseconds. The last image and inset show an overlay of the mRNP path that is color coded based on position (white, nuclear docked; yellow, transition; blue, cytoplasmic docked/cytoplasm). Each green arrowhead and star denotes mRNP positions at the beginning and end of the track, respectively. Bars, 1 µm.