Figure 1.
Figure 1. REF strain design and characterization. (A) Schematic displaying features of the yeast strains used to monitor mRNP export. Upon transcription, the GFA1 mRNA that carries 24xPP7 loops in the 3′ UTR is bound by the PP7-CP-3xYFP appearing as particles that can be tracked in relation to NPCs that are marked by Ndc1-tdTomato. (B) Fluorescent images of the Ndc1-tdTomato signal in REF cells using identical image acquisition settings showing the improvement in image quality after removal of the yeast cell wall. Examples of both raw and Laplacian filtered images are shown. Bar, 1 µm. (C) Dot plot displaying the localization precision (pixel = 96 nm) obtained when tracking mRNP particles in cells with (−sphero; n = 86) and without a cell wall (+sphero; n = 156), with the mean denoted by a black line for each.

REF strain design and characterization. (A) Schematic displaying features of the yeast strains used to monitor mRNP export. Upon transcription, the GFA1 mRNA that carries 24xPP7 loops in the 3′ UTR is bound by the PP7-CP-3xYFP appearing as particles that can be tracked in relation to NPCs that are marked by Ndc1-tdTomato. (B) Fluorescent images of the Ndc1-tdTomato signal in REF cells using identical image acquisition settings showing the improvement in image quality after removal of the yeast cell wall. Examples of both raw and Laplacian filtered images are shown. Bar, 1 µm. (C) Dot plot displaying the localization precision (pixel = 96 nm) obtained when tracking mRNP particles in cells with (−sphero; n = 86) and without a cell wall (+sphero; n = 156), with the mean denoted by a black line for each.

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