Analysis of spindles and Asp localization in NBs. (A) asp^t25/Df NBs expressing indicated transgenes were fixed and stained for β-Tubulin, pH3, and centrosomin (Cnn). Outline denotes NB cortex, and arrowheads mark centrosome position. (B) Snapshots from live movie of indicated genotype. Localization of Asp is similar to samples in A, except here the weak Asp^C localization to spindle MTs is clear (yellow arrowheads). Bars, 2 µm.