CaM localization and dynamics in NBs. (A) Prophase NBs from larvae expressing GFP-CaM stained for centrosomin (Cnn) and β-tubulin. (A′) Close up of yellow boxed region in A and line scan along the yellow line for CaM, Cnn, and Tubulin. (B) As described for A, but the cell is in metaphase. (B′) As described for A′. Note the difference in CaM localization relative to the centrosome between prophase (A) and metaphase (B). C, centrosome; P, pole. (C) Live cell imaging of mitotic NB (dotted outline) expressing GFP-CaM (Video 2). (C′) Inset of red boxed region in C. Arrows denote spindle foci. P, pole. (D) Still frames from metaphase NB (Video 3), with arrowhead denoting GFP-CaM foci moving toward the pole. Note the increase in signal intensity at pole as time progresses. (D′) Average intensity projection of metaphase (left panel) showing position of kymographs (right). Arrowheads denote foci movement. Bars: (A and B) 5 µm; (A’ and B’) 1 µm; (C) 10 µm; (C’, D, and D’) 2 µm.