Asp and CaM interact to form a complex that streams along spindles. (A) Mitochondria targeting assay for each of the indicated Asp fragments. White dotted line corresponds to cell outline, and yellow dotted lines show nuclei. Bar, 2 µm. (B) Yeast two-hybrid analysis of Asp constructs and CaM. Left column indicates growth, and right column indicates interaction. (C) Single frame from Video 1 of live S2 cell expressing RFP-CaM and GFP-Asp. Boxed region (yellow) denotes inset, bottom panel. Colored lines represent position of kymograph in E. Arrowheads denote colocalized foci. Bar, 5 µm; inset, 1 µm. (D) 2D histograms from colocalization analysis. (E) Kymograph along positions denoted in C. Time bar, 1 min; distance bar, 2.5 µm. (F) Histogram of movement rates for CaM and Asp (µm/min). n > 200 tracks from more than nine cells.