Figure 3.
![Figure 3. The middle region of Asp has MT cross-linking activity. (A) Rescue experiments using UTR RNAi and ectopic expression of GFP fusion proteins (n = 23–32). (B) Expression of Asp [574–972 aa]-GFP resulted in overbundling of spindle MTs and unfocused the poles. (C) Purified Asp [574–972 aa]-GFP demonstrated MT cross-linking activity in vitro. Taxol-stabilized, rhodamine-labeled MTs were mixed with (top) or without (bottom) Asp [574–972 aa]-GFP. We could not eliminate a smaller size band, possibly a degraded product of Asp [574–972 aa]-GFP. Bars: (A and B) 5 µm; (C) 10 µm.](https://cdn.rupress.org/rup/content_public/journal/jcb/211/5/10.1083_jcb.201507001/7/jcb_201507001_fig3.jpeg?Expires=1771592718&Signature=Fo0MhiF1SHX7KIUmiij1THSxgYuG08QOEetVgBXNDP~iWYQhR6NhICm44tlm8HMAVxldN7TxadVlkrov~xt7sVJ9oOBadmP7zzH2J7YXLTNQdcX64Tt~Vzt~EOjEfi16gyRZMVEFK2fsJwSE47OqJ4Zje4quM4wxw5ugaEch7-7OAdkYBBBJy8~kdXjKjJuAhSFA31ninuqLxrTmwj~A0f6MkZY1xYyvsj~bllrl6-gEp8iol-OxbDnpAvHXoqqidOVmWwkbqB036JCg5QjmfzZZOeyim6EsPmiLJaqMrky9eWE9zgR~mAqFKKsCkSKyo8-raDldca69QcF3kO5LhA__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
The middle region of Asp has MT cross-linking activity. (A) Rescue experiments using UTR RNAi and ectopic expression of GFP fusion proteins (n = 23–32). (B) Expression of Asp [574–972 aa]-GFP resulted in overbundling of spindle MTs and unfocused the poles. (C) Purified Asp [574–972 aa]-GFP demonstrated MT cross-linking activity in vitro. Taxol-stabilized, rhodamine-labeled MTs were mixed with (top) or without (bottom) Asp [574–972 aa]-GFP. We could not eliminate a smaller size band, possibly a degraded product of Asp [574–972 aa]-GFP. Bars: (A and B) 5 µm; (C) 10 µm.
