Asp functions independently of Ncd in acentrosomal pole focusing. (A) Quantification of spindle pole width (left), spindle width (middle), and their relative values (right) after Asp or Ncd RNAi in the absence of centrosomes. Error bars indicate SEM (n = 18–54). Bar, 10 µm. (B) Frequency of V-shaped MTs after Cnn/Asp (n = 32) or Cnn/Ncd (n = 38) RNAi. (C) Ectopic Ncd-GFP expression did not suppress the pole-unfocusing phenotype of Asp RNAi (n = 23; control n = 13). (D) Ectopic Asp-GFP expression (low and high expression levels) did not suppress the pole-unfocusing phenotype of Ncd RNAi (n = 25). (E and F) Asp-GFP and mCherry-tubulin were imaged in the presence or absence of Ncd. The cells were depleted of centrosomes using Cnn RNAi. In both cases, Asp-GFP was detected at the focused pole at prometaphase (arrowheads). Note that the right-side pole in F was out of microscopic focus. A magnification view is also displayed. Bars: (C–F) 5 µm.