Motility of mitochondria does not depend on EEs. (A) Distribution of mitochondria, labeled with Lga1-GFP, in U. maydis. 2D-deconvolved maximum projection of a Z-axis stack, adjusted in brightness, contrast, and gamma settings. Bar, 5 µm. (B) Contrast-inverted kymograph showing the dynamic behavior of mitochondria. The organelles show rare short-range motility (arrowhead). Bars, 3 s, 2 µm. (C) EE, PO, LD and mitochondria (Mitos) motility in U. maydis, given as the percentage of organelles that show motility within 15s observation time. Note that POs, LDs and mitochondria switch between stationary and directed transport, whereas EEs are constantly moving. All bars are mean ± SEM (n = 30 cells, two experiments). ***, P < 0.0001 versus control (Student’s t test). No difference in mitochondria motility was found in Δhok1 mutants (P = 0.2430; Student’s t test). (D) Velocity of bidirectionally moving mitochondria, LDs, and POs. Bars are mean ± SEM (n = 58 cells, two experiments). ***, P < 0.0001 (Student’s t test). P-value for nonsignificant difference shown above bars. (E) Mitochondria in a Δhok1 cell. 2D-deconvolved maximum projection of a Z-axis stack, adjusted in brightness, contrast, and gamma settings. Bar, 5 µm. (F) Fluorescent intensity profiles of mitochondria, labeled with Lga1-GFP, in control and Δhok1 mutant cells. The location of the hyphal tips is indicated (Tip). Each data point represents the mean ± SEM of 20 cells.