EEs drive LD motility. (A) LDs in U. maydis. 2D-deconvolved maximum projection of a Z-axis stack, adjusted in brightness, contrast, and gamma settings. Bar, 5 µm. (B) Dynamic behavior of LDs. Organelles are stationary (blue arrowhead) or undergo random motility. Occasionally, directed long-range motility occurs (red arrowhead). Image was contrast inverted. Bars, 3 s, 2 µm. See Video 4. (C) Flux of LD motility in the presence of DMSO (control) or benomyl, and in Δhok1. LD motility depends on MTs and the EE-specific motor adapter. All bars are mean ± SEM (n = 40 cells, 2 experiments). ***, P < 0.0001 versus control (Student’s t test). (D) LDs in a Δhok1. 2D-deconvolved maximum projection of a Z-axis stack, adjusted in brightness, contrast, and gamma settings. Bar, 5 µm. (E) Fluorescent intensity profiles of Erg6-GFP in control and Δhok1 cells. Position of cell tips is indicated (Tip). Each data point represents the mean ± SEM (20 cells, two experiments). (F) Co-motility of EEs, labeled with mCherry-Rab5a (red) and LDs, labeled with Erg6-GFP (green). Background interference was reduced by photobleaching (red arrow). Image series covers ∼1.55 s and is adjusted in brightness, contrast, and gamma settings. Bar, 1 µm. See Video 5. (G) Intensity profiles of a co-migrating LD (red) and EEs (blue line). Direction of transport indicated by green arrow. Data points are mean ± SEM (n = 37–54 cells, three experiments).