POs move along MTs in U. m a ydis. (A) POs in U. maydis. 2D-deconvolved maximum projection of a Z-axis stack, adjusted in brightness, contrast and gamma settings. Bar, 5 µm. (B) Dynamics of POs. POs are stationary (blue arrowheads), undergo short motions (green arrowheads), or switch to directed motility (red arrowheads). Images were contrast inverted and adjusted in brightness, contrast, and gamma settings. Bar, 1 µm. Time indicated in lower right corner. (C) Motility of POs. Most POs show random motion (blue arrowhead); few undergo directed motility (red arrowheads). Image contrast inverted. Bars, 5 s, 2 µm. See Video 1. (D) Flux, run length, and velocity of bidirectionally moving POs. Bars are mean ± SEM (n = 30–69 cells, two experiments). P-values of Student’s t tests shown above the bars. (E) Motility of POs in cells treated with DMSO, benomyl, or latrunculin A (LatA). PO motility stops when MTs are disrupted. Image contrast inverted. Bars, 3 s, 3 µm. (F) Motility of a PO (red; arrowhead) along a MT (green). Image series covers 6.16 s. Images adjusted in brightness, contrast, and gamma settings. Bar, 2 µm. See Video 2.