Figure 5.
Figure 5. Short-term depletion of Pex1 affects matrix protein import but not PMP transport. (A) Western blot analysis of Pex1-HA-AID levels in cells as grown in <0–90 min after addition of 0.5-mM auxin. (B) Pex1-HA-AID cells expressing the peroxisomal matrix marker HcRed-PTS1 were transformed with various PMP-GFP expression plasmids driven by the GAL1 promoter. Cells were grown to log phase on raffinose medium, and auxin was added to 0.5 mM. 45 min later, cells were resuspended in a galactose medium + auxin for 15 min (Pex3, Pex11, and Pex13) or 45 min (Pex10) to induce PMP-GFP expression and imaged by epifluorescence microscopy. (C) WT cells expressing HcRed-PTS1 constitutively and Pex11- or Pex13-GFP from the GAL1 promoter were grown as described in B in the presence of auxin. Bars, 5 µm.

Short-term depletion of Pex1 affects matrix protein import but not PMP transport. (A) Western blot analysis of Pex1-HA-AID levels in cells as grown in <0–90 min after addition of 0.5-mM auxin. (B) Pex1-HA-AID cells expressing the peroxisomal matrix marker HcRed-PTS1 were transformed with various PMP-GFP expression plasmids driven by the GAL1 promoter. Cells were grown to log phase on raffinose medium, and auxin was added to 0.5 mM. 45 min later, cells were resuspended in a galactose medium + auxin for 15 min (Pex3, Pex11, and Pex13) or 45 min (Pex10) to induce PMP-GFP expression and imaged by epifluorescence microscopy. (C) WT cells expressing HcRed-PTS1 constitutively and Pex11- or Pex13-GFP from the GAL1 promoter were grown as described in B in the presence of auxin. Bars, 5 µm.

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