Figure 4.
Figure 4. Loss of mitochondria in Mffgt myocardium. (A) Low-magnification EM showing differences in mitochondrial mass. Bars, 5 µm. (B) Quantification of mitochondrial area in EM images. (C) HSP60/actin protein ratio, normalized to wild type (n ≥ 7). (D) mtDNA per cell, normalized to wild-type content (n ≥ 7). (E) EM of mutant myocardium, showing examples of defective mitochondria. Arrow indicates mitophagic structure. Bars, 0.5 µm. (F) Heart sections immunostained with LC3 or p62 (red) and HSP60 (green). Arrows indicate positive puncta. Mutant inset is enlarged. Bars: 10 µm; (mutant inset) 50 µm. Error bars = SEM. P-values as compared with wild type: ^, P ≤ 0.05; *, P ≤ 0.01; **, P ≤ 0.001. nDNA, nuclear DNA; WT, wild type.

Loss of mitochondria in Mffgt myocardium. (A) Low-magnification EM showing differences in mitochondrial mass. Bars, 5 µm. (B) Quantification of mitochondrial area in EM images. (C) HSP60/actin protein ratio, normalized to wild type (n ≥ 7). (D) mtDNA per cell, normalized to wild-type content (n ≥ 7). (E) EM of mutant myocardium, showing examples of defective mitochondria. Arrow indicates mitophagic structure. Bars, 0.5 µm. (F) Heart sections immunostained with LC3 or p62 (red) and HSP60 (green). Arrows indicate positive puncta. Mutant inset is enlarged. Bars: 10 µm; (mutant inset) 50 µm. Error bars = SEM. P-values as compared with wild type: ^, P ≤ 0.05; *, P ≤ 0.01; **, P ≤ 0.001. nDNA, nuclear DNA; WT, wild type.

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