Figure 1. Cardiomyopathy in Mff gt mice.... | Rockefeller University Press

Figure 1.

$Figure 1. Cardiomyopathy in Mffgt mice. (A) Survival curve. Mffgt mutant (mut) mice (n = 22) die by 13 wk on average, as opposed to wild-type controls (n = 14). (B) Increased size, dilation of chambers, and thinning of walls in mutant hearts. (C) Heart mass normalized to tibia length. (D) Masson’s trichrome stain of heart sections. Arrowheads indicate fibroblasts. Blue strands (arrows) denote collagen deposition. (E) TUNEL staining of heart sections. Arrows indicate apoptotic nuclei. (F) Quantification of apoptotic nuclei per field (n ≥ 11). (G) Heart rate (n ≥ 6). (H) M-mode echocardiogram of 13–14-wk-old mice. Electrocardiogram is superimposed. (I and J) Longitudinal echocardiography measuring fractional shortening (I) and ejection fraction (J). Study conducted on 5 wild-type and 10 Mffgt mice (five mutants died during the course of the study). Error bars = SEM. *, P ≤ 0.01; **, P ≤ 0.001. Bars, 50 µm. WT, wild type.$

Cardiomyopathy in Mff^gt mice. (A) Survival curve. Mff^gt mutant (mut) mice (n = 22) die by 13 wk on average, as opposed to wild-type controls (n = 14). (B) Increased size, dilation of chambers, and thinning of walls in mutant hearts. (C) Heart mass normalized to tibia length. (D) Masson’s trichrome stain of heart sections. Arrowheads indicate fibroblasts. Blue strands (arrows) denote collagen deposition. (E) TUNEL staining of heart sections. Arrows indicate apoptotic nuclei. (F) Quantification of apoptotic nuclei per field (n ≥ 11). (G) Heart rate (n ≥ 6). (H) M-mode echocardiogram of 13–14-wk-old mice. Electrocardiogram is superimposed. (I and J) Longitudinal echocardiography measuring fractional shortening (I) and ejection fraction (J). Study conducted on 5 wild-type and 10 Mff^gt mice (five mutants died during the course of the study). Error bars = SEM. *, P ≤ 0.01; **, P ≤ 0.001. Bars, 50 µm. WT, wild type.