Mitotic swelling is observed for a large set of cell lines. (A) Phase and FXm images of a L929 cell, and mean volume of fibroblastic L929 cells undergoing mitosis (n = 38). (B) Phase and FXm images of an adherent HT29 cell, and mean volume of HT29 cells undergoing mitosis (n = 16). (C) Phase and FXm images of a floating Raji cell, and mean volume of Raji cells undergoing mitosis (n = 67). For A–C, time is set to 0 as cells start to round up, and the gray area displays the SEM. (D) Relative increase of volume in mitosis for different cell lines. For each cell, the volume increase is taken between mitotic entry and the maximal volume reached during mitosis. Cells were divided into three categories, from solid tissue with spread or rounded morphology and from blood with a rounded morphology. (E and F) Relative and absolute volume overshoot per cell line as a function of their rounding volume at mitotic entry. The gray line in F shows the linear regression (R2 = 0.63). Two-tailed t test: P = 0.49 (E) and P = 0.01 (F).
