Figure 7.
Figure 7. Destabilized ER–LD contact sites in Seipin complex mutants. (A) 2D tomograms derived from 250-nm-thick sections of wt cells. Magnified insets on the right of each tomogram show ER–LD contact sites (arrowhead). Bars, 200 nm. (B) 2D tomograms derived from a 250-nm-thick sections of ldb16Δ cells. Magnified insets on the right of each tomogram show the contact sites between ER and supersized LDs (arrowhead). Bars, 200 nm. (C) 2D tomograms derived from a 250-nm-thick sections of ldb16Δ cells. Arrows indicate ER regions with enlarged luminal width. Bars, 200 nm. (D) Electron micrographs showing immunolocalization of endogenously expressed Ldb16-GFP in wt cells. Arrows point to 12-nm immunogold particles labeling specifically Ldb16-GFP. Bars, 100 nm. C, cytoplasm; N, nucleus.

Destabilized ER–LD contact sites in Seipin complex mutants. (A) 2D tomograms derived from 250-nm-thick sections of wt cells. Magnified insets on the right of each tomogram show ER–LD contact sites (arrowhead). Bars, 200 nm. (B) 2D tomograms derived from a 250-nm-thick sections of ldb16Δ cells. Magnified insets on the right of each tomogram show the contact sites between ER and supersized LDs (arrowhead). Bars, 200 nm. (C) 2D tomograms derived from a 250-nm-thick sections of ldb16Δ cells. Arrows indicate ER regions with enlarged luminal width. Bars, 200 nm. (D) Electron micrographs showing immunolocalization of endogenously expressed Ldb16-GFP in wt cells. Arrows point to 12-nm immunogold particles labeling specifically Ldb16-GFP. Bars, 100 nm. C, cytoplasm; N, nucleus.

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