Relocalization of amphipathic helix-containing proteins in seipin complex mutants. (A) Localization of GFP-tagged Kes1, Pct1, Gvp36, and the corresponding counterparts lacking the AH Kes1^Δ2–29, Pct1^Δ261–282, and Gvp36^Δ2–35 in cells with the indicated genotype. The GFP fusion proteins were expressed from a centromeric plasmid. Cells in early stationary phase grown in SC media (I−) or SC supplemented with 75 µM inositol (I+) were imaged. LDs were stained with the neutral lipid dye MDH. Bar, 5 µm. On the right the percentage of cells with the indicated genotype displaying abnormal foci of GFP-tagged proteins. The mean of three independent experiments is displayed; error bars represent SD. At least 100 cells/experiment were analyzed per genotype. (B) Localization of Kes1(1–38)-GFP and Gvp36(1–50)-GFP in cells with the indicated genotype grown in SC supplemented with inositol. Kes1(1–38)-GFP and Gvp36(1–50)-GFP were expressed from the endogenous loci and encode for AH of Kes1 and Gvp36, respectively. LDs were stained with the neutral lipid dye MDH. Bar, 5 µm.