Lipid-driven relocalization of Opi1 in Fld1/Ldb16 complex mutants. (A) Localization of Opi1 in wt and ldb16Δ cells grown in SC media to early stationary phase. Opi1 was expressed from the endogenous locus as C-terminal mCherry fusion (Opi1-Cherry). Nuclear envelope was labeled by endogenously expressed Hmg1-GFP. LDs were stained with the neutral lipid dye MDH. Yellow arrowheads indicate supersized LDs; white arrowhead indicates LD aggregates. Bar, 5 µm. (B) Localization of endogenously expressed Opi1-GFP in cells with the indicated genotype grown to early stationary phase in SC media. LDs were stained with the neutral lipid dye MDH. Bar, 5 µm. (C) Localization of GFP-Spo20^51–91 expressed from a 2 µm plasmid in cells with the indicated genotype. LDs were stained with the neutral lipid dye MDH. Bar, 5 µm. (D) Percentage of cells with the indicated genotype displaying abnormal foci of GFP-Spo20^51–91. Late logarithmic cells grown in SC media (−) or SC supplemented with 75 µM inositol (+) are shown. The mean of two independent experiments is graphed; error bars represent SD. For each genotype and condition, at least 100 cells/experiment were analyzed.